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1.
Cellulose (Lond) ; 31(5): 3129-3142, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38577421

RESUMO

The research conducted on kraft cooking of for different chip sizes is often not representative for the industrial process since the chip size fractions were made of high-quality wood without impurities. We evaluated the effects and the potential of cooking non ideal spruce chip fractions after industrial chipping and screening. The chips were classified according to SCAN 40:01, and the respective fractions were cooked under the identical conditions to mimic the effect of a joint cooking in the industrial digester. For the undersized chips we found higher bark content, a lower screened yield, a higher Kappa number, lower fiber length and lower tensile strength. For the oversized chips, the fiber length and tensile index were also considerably lower. A lower wood quality due to high knot content in the larger fractions was found to be the reason for that. Based on the data obtained from the experiments and literature, different process options for increased yield and reduced chemical consumption are discussed, e.g., separate cooking of different chip fractions. Improved chip screening seems to be the process improvement with lowest costs and highest impact.

2.
J Exp Biol ; 227(6)2024 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-38357776

RESUMO

A skeletal muscle's peak force production and excursion are based on its architectural properties that are, in turn, determined by its mass, muscle fiber length and physiological cross-sectional area (PCSA). In the classic interspecific study of mammalian muscle scaling, it was demonstrated that muscle mass scales positively allometrically with body mass whereas fiber length scales isometrically with body mass, indicating that larger mammals have stronger leg muscles than they would if they were geometrically similar to smaller ones. Although this relationship is highly significant across species, there has never been a detailed intraspecific architectural scaling study. We have thus created a large dataset of 896 muscles across 34 human lower extremities (18 females and 16 males) with a size range including approximately 90% and 70% of the United States population height and mass, respectively, across the range 36-103 years. Our purpose was to quantify the scaling relationships between human muscle architectural properties and body size. We found that human muscles depart greatly from isometric scaling because muscle mass scales with body mass1.3 (larger exponent than isometric scaling of 1.0) and muscle fiber length scales with negative allometry with body mass0.1 (smaller exponent than isometric scaling of 0.33). Based on the known relationship between architecture and function, these results suggest that human muscles place a premium on muscle force production (mass and PCSA) at the expense of muscle excursion (fiber length) with increasing body size, which has implications for understanding human muscle design as well as biomechanical modeling.


Assuntos
Perna (Membro) , Músculo Esquelético , Humanos , Animais , Masculino , Feminino , Músculo Esquelético/fisiologia , Fibras Musculares Esqueléticas/fisiologia , Tamanho Corporal , Mamíferos , Extremidade Inferior
3.
Neurophysiol Clin ; 54(4): 102955, 2024 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-38422588

RESUMO

OBJECTIVES: Corneal confocal microscopy (CCM) is a non-invasive technique that examines the corneal cellular structure. Its use in the detection of small fiber neuropathy is being researched. In our study, we examined the role of CCM in the detection of small fiber neuropathy in diabetic patients, as well as the differences between CCM findings in diabetic patients with and without overt polyneuropathy with neuropathic symptoms. METHODS: 56 Diabetes Mellitus (DM) patients and 18 healthy controls were included in the study. The individuals included in the study were divided into three groups. Patients with diabetes who were found to have polyneuropathy according to electrophysiological diagnostic criteria were classified as Group 1, patients with diabetes and neuropathic symptoms without overt polyneuropathy according to electrophysiological diagnostic criteria were classified as Group 2, and healthy individuals were classified as Group 3. Electrophysiological examination and corneal imaging with CCM were performed in all groups. RESULTS: The CNFD and CNFL values of individuals in the diabetic group were discovered to be lower. CNFD values differ statistically between the groups (p = 0.047). Group 1-Group 3 differs from Group 2-Group 3 (respectively; p = 0.018, p = 0.048). CONCLUSION: Our study demonstrates that CCM can be used in patients with neuropathic symptoms and no polyneuropathy detected in EMG and thought to have small fiber neuropathy. CCM provides an opportunity for early diagnosis in small fiber neuropathy.

4.
Am J Physiol Cell Physiol ; 326(3): C749-C755, 2024 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-38189131

RESUMO

Experimental techniques in single human skeletal muscle cells require manual dissection. Unlike other mammalian species, human skeletal muscle is characterized by a heterogeneous mixture of myosin heavy chain (MHC) isoforms, typically used to define "fiber type," which profoundly influences cellular function. Therefore, it is beneficial to predict MHC isoform at the time of dissection, facilitating a more balanced fiber-type distribution from a potentially imbalanced sample. Although researchers performing single fiber dissection report predicting fiber-type based on mechanical properties of fibers upon dissection, a rigorous examination of this approach has not been performed. Therefore, we measured normalized fiber length (expressed as a % of the length of the bundle from which the fiber was dissected) in single fibers immediately following dissection. Six hundred sixty-eight individual fibers were dissected from muscle tissue samples from healthy, young adults to assess whether this characteristic could differentiate fibers containing MHC I ("slow" fiber type) or not ("fast" fiber type). Using receiver operator characteristic (ROC) curves, we found that differences in normalized fiber length (114 ± 13%, MHC I; 124 ± 17%, MHC IIA, P < 0.01) could be used to predict fiber type with excellent reliability (area under the curve = 0.72). We extended these analyses to include older adults (2 females, 1 male) to demonstrate the durability of this approach in fibers with likely different morphology and mechanical characteristics. We report that MHC isoform expression in human skeletal muscle fibers can be predicted at the time of dissection, regardless of origin.NEW & NOTEWORTHY A priori estimation of myosin heavy chain (MHC) isoform in individual muscle fibers may bias the relative abundance of fiber types in subsequent assessment. Until now, no standardized assessment approach has been proposed to characterize fibers at the time of dissection. We demonstrate an approach based on normalized fiber length that may dramatically bias a sample toward slow twitch (MHC I) or fast twitch (not MHC I) fiber populations.


Assuntos
Fibras Musculares Esqueléticas , Cadeias Pesadas de Miosina , Animais , Feminino , Adulto Jovem , Humanos , Masculino , Idoso , Cadeias Pesadas de Miosina/metabolismo , Reprodutibilidade dos Testes , Fibras Musculares Esqueléticas/metabolismo , Músculo Esquelético/metabolismo , Isoformas de Proteínas/metabolismo , Mamíferos/metabolismo
5.
Polymers (Basel) ; 16(1)2024 Jan 04.
Artigo em Inglês | MEDLINE | ID: mdl-38201825

RESUMO

The tensile strength and modulus of elasticity of a jute/polylactic acid (PLA) composite were found to vary nonlinearly with the loading angle of the specimen through the tensile test. The variation in these properties was related to the fiber orientation distribution (FOD) and fiber length distribution (FLD). In order to study the effects of the FOD and FLD of short fibers on the mechanical properties and to better predict the mechanical properties of short-fiber composites, the true distribution of short fibers in the composite was accurately obtained using X-ray computed tomography (XCT), in which about 70% of the jute fibers were less than 300 µm in length and the fibers were mainly distributed along the direction of mold flow. The probability density functions of the FOD and FLD were obtained by further analyzing the XCT data. Strength and elastic modulus prediction models applicable to short-fiber-reinforced polymer (SFRP) composites were created by modifying the laminate theory and the rule of mixtures using the probability density functions of the FOD and FLD. The experimental measurements were in good agreement with the model predictions.

6.
Int J Biol Macromol ; 256(Pt 2): 128036, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37972829

RESUMO

Cotton is the most economically important natural fiber crop grown in more than sixty-five countries of the world. Fiber length is the main factor affecting fiber quality, but the existing main varieties are short in length and cannot suit the higher demands of the textile industry. It is necessary to discover functional genes that enable fiber length improvement in cotton through molecular breeding. In this study, overexpression of GhEB1C in Arabidopsis thaliana significantly promotes trichomes, tap roots, and root hairs elongation. The molecular regulation of GhEB1C involves its interactions with itself and GhB'ETA, and the function of GhEB1C regulation mainly depends on the two cysteine residues located at the C-terminal. In particular, the function activity of GhEB1C protein triggered with the regulation of protein phosphatase 2A, while silencing of GhEB1C in cotton significantly influenced the fiber protrusions and elongation mechanisms., Further, influenced the expression of MYB-bHLH-WD40 complex, brassinosteroids, and jasmonic acid-related genes, which showed that transcriptional regulation of GhEB1C is indispensable for cotton fiber formation and elongation processes. Our study analyzed the brief molecular mechanism of GhEB1C regulation. Further elucidated that GhEB1C can be a potential target gene to improve cotton fiber length through transgenic breeding.


Assuntos
Arabidopsis , Gossypium , Gossypium/genética , Gossypium/metabolismo , Proteína Fosfatase 2/metabolismo , Melhoramento Vegetal , Fibra de Algodão , Arabidopsis/genética , Arabidopsis/metabolismo , Raízes de Plantas/metabolismo , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo
7.
Front Plant Sci ; 14: 1254103, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37662180

RESUMO

Introduction: Phosphorus (P) deficiency hinders cotton (Gossypium hirustum L.) growth and development, seriously affecting lint yield and fiber quality. However, it is still unclear how P fertilizer affects fiber length. Methods: Therefore, a two-year (2019-2020) pool-culture experiment was conducted using the split-plot design, with two cotton cultivars (CCRI-79; low-P tolerant and SCRC-28; low-P sensitive) as the main plot. Three soil available phosphorus (AP) contents (P0: 3 ± 0.5, P1: 6 ± 0.5, and P2 (control) with 15 ± 0.5 mg kg-1) were applied to the plots, as the subplot, to investigate the impact of soil AP content on cotton fiber elongation and length. Results: Low soil AP (P0 and P1) decreased the contents of the osmotically active solutes in the cotton fibers, including potassium ions (K+), malate, soluble sugar, and sucrose, by 2.2-10.2%, 14.4-47.3%, 8.7-24.5%, and 10.1-23.4%, respectively, inhibiting the vacuoles from facilitating fiber elongation through osmoregulation. Moreover, soil AP deficiency also reduced the activities of enzymes participated in fiber elongation (plasma membrane H+-ATPase (PM-H+-ATPase), vacuole membrane H+-ATPase (V-H+-ATPase), vacuole membrane H+-translocating inorganic pyrophosphatase (V-H+-PPase), and phosphoenolpyruvate carboxylase (PEPC)). The PM-H+-ATPase, V-H+-ATPase, V-H+-PPase, and PEPC were reduced by 8.4-33.0%, 7.0-33.8%, 14.1-38.4%, and 16.9-40.2%, respectively, inhibiting the transmembrane transport of the osmotically active solutes and acidified conditions for fiber cell wall, thus limiting the fiber elongation. Similarly, soil AP deficiency reduced the fiber length by 0.6-3.0 mm, mainly due to the 3.8-16.3% reduction of the maximum velocity of fiber elongation (VLmax). Additionally, the upper fruiting branch positions (FB10-11) had higher VLmax and longer fiber lengths under low soil AP. Discussion: Cotton fibers with higher malate content and V-H+-ATPase and V-H+-PPase activities yielded longer fibers. And the malate and soluble sugar contents and V-H+-ATPase and PEPC activities in the SCRC-28's fiber were more sensitive to soil AP deficiency in contrast to those of CCRI-79, possibly explaining the SCRC-28 fiber length sensitivity to low soil AP.

8.
Materials (Basel) ; 16(17)2023 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-37687662

RESUMO

In order to improve the mechanical properties and deformation characteristics of permeable concrete, glass fiber was added to this type of concrete. Based on an unconfined compressive strength test, non-contact full-field strain measurement system, and scanning electron microscopy test, the effects of aggregate particle composition, shaking time, fly ash content, fiber length, and fiber content on the strength and permeability of permeable concrete were studied. The results show that the strength and water permeability of permeable concrete are negatively correlated with an increase in shaking time. When the aggregate particle size is 5-10 mm, the permeable concrete has both good strength and permeability. Proper incorporation of fly ash improves the compactness inside the structure. The influence of different lengths of glass fiber on the strength of permeable concrete first increases and then decreases, and the permeable property decreases. With the same fiber length, the strength increases first and then decreases with an increase in the content, while the porosity and water permeability coefficient decrease. Under the test conditions, when the length of glass fiber is 6 mm, and the dosage is 2 kg/m3, the strength performance of permeable concrete is the best, and the permeability effect is good at the same time.

9.
Animals (Basel) ; 13(13)2023 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-37443977

RESUMO

Maintaining meat quality is essential to sustainable livestock management. Therefore, identifying alternative feed materials while considering consumer acceptance is crucial. So, the aim of this study was to evaluate the effect of C. glomerata-biomass-supplemented feeds on rabbit muscles' physical properties, sensory profiles, and evaluators' emotional responses to them. A total of thirty 52-day-old weaned Californian breed rabbits were randomly allocated to one of three dietary treatments: standard compound diet (SCD), SCD supplemented with 4% C. glomerata (CG4), or SCD supplemented with 8% C. glomerata (CG8). After the 122-day-old rabbits were slaughtered, post-mortem dissection of the rabbit Longissimus dorsi (LD) and hind leg (HL) muscles was conducted. The physical and histomorphometric features, sensory analyses, and emotional responses to the rabbit's muscles were determined. Study results revealed CG4 and CG8 treatments significantly increased rabbit muscle moisture, while CG8 increased cooking losses in HL muscles (p < 0.05). Moreover, both CG treatments reduced the darkness and redness of fresh and cooked rabbit muscles compared to SCD (p < 0.05). CG8 treatment compared to SCD resulted in longer LD muscle fibers (p < 0.05). Evaluators discovered that the average scores for each sensory description of rabbit meat are acceptable and that consuming CG8-HL muscles can increase happiness based on emotional responses. Consequently, replacing traditional feed materials in rabbit feed with C. glomerata can lead to not only more sustainable production but also more consumer-acceptable rabbit meat.

10.
Polymers (Basel) ; 15(9)2023 Apr 25.
Artigo em Inglês | MEDLINE | ID: mdl-37177191

RESUMO

Long-fiber thermoplastic (LFT) materials compounded via the direct LFT (LFT-D) process are very versatile composites in which polymers and continuous reinforcement fiber can be combined in almost any way. Polycarbonate (PC) as an amorphous thermoplastic matrix system reinforced with glass fibers (GFs) is a promising addition regarding the current development needs, for example battery enclosures for electromobility. Two approaches to the processing and compression molding of PC GF LFT-D materials with various parameter combinations of screw speed and fiber rovings are presented. The resulting fiber lengths averaged around 0.5 mm for all settings. The tensile, bending, Charpy, and impact properties were characterized and discussed in detail. Special attention to the characteristic charge and flow area formed by compression molding of LFT-D materials, as well as sample orientation was given. The tensile modulus was 10 GPa, while the strength surpassed 125 MPa. The flexural modulus can reach up to 11 GPa, and the flexural strength reached up to 216 MPa. PC GF LFT-D is a viable addition to the LFT-D process, exhibiting good mechanical properties and stable processability.

11.
Polymers (Basel) ; 15(8)2023 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-37112071

RESUMO

The increasing demand for renewable raw materials and lightweight composites leads to an increasing request for natural fiber composites (NFC) in series production. In order to be able to use NFC competitively, they must also be processable with hot runner systems in injection molding series production. For this reason, the influences of two hot runner systems on the structural and mechanical properties of Polypropylene with 20 wt.% regenerated cellulose fibers (RCF) were investigated. Therefore, the material was processed into test specimens using two different hot runner systems (open and valve gate) and six different process settings. The tensile tests carried out showed very good strength for both hot runner systems, which were max. 20% below the reference specimen processed with a cold runner and, however, significantly influenced by the different parameter settings. Fiber length measurements with the dynamic image analysis showed approx. 20% lower median values of GF and 5% lower of RCF through the processing with both hot runner systems compared to the reference, although the influence of the parameter settings was small. The X-ray microtomography performed on the open hot runner samples showed the influences of the parameter settings on the fiber orientation. In summary, it was shown that RCF composites can be processed with different hot runner systems in a wide process window. Nevertheless, the specimens of the setting with the lowest applied thermal load showed the best mechanical properties for both hot runner systems. It was furthermore shown that the resulting mechanical properties of the composites are not only due to one structural property (fiber length, orientation, or thermally induced changes in fiber properties) but are based on a combination of several material- and process-related properties.

12.
Int J Artif Organs ; 46(4): 202-208, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-36907998

RESUMO

Continuous renal replacement therapy (CRRT) has become the most commonly used acute blood purification therapy for critically ill patients. As a key point of extracorporeal blood circulation, the CRRT filter plays a decisive role in therapeutic efficacy. However, few in vitro studies have been conducted on CRRT filters, particularly concerning the effects of design factors on filter effectiveness and safety profile; no comprehensive evaluation system has been established. Here, we designed nine CRRT filters with various combinations of hollow fiber packing density (PD) and housing shape (effective hollow fiber length (L) and inner housing diameter (D) ratio (L/D ratio)) and introduced a high-frequency sampling pressure monitor to accurately monitor small changes in transmembrane pressure (TMP) and ultrafiltration rate (UFR) over time. We also used concentration polarization mass transfer resistance (Rc), change in sieving coefficient (S) of albumin over time, and amount of albumin removed (Mfld) to investigate the effects of two design factors on albumin filtration performance and analyze the mechanism of protein filtration performance over time, thereby establishing a comprehensive in vitro evaluation system to explore the safety profile of CRRT filters. Our results showed that the nine CRRT filters designed with different combinations of PD (50%, 55%, and 60%) and L/D ratio (2.9, 5.3, and 9.3) were able to maintain stability in terms of hemodynamics and water permeability; the lowest Mfld was PD = 60% and L/D ratio = 9.3, which indicates that design factor optimization can effectively control albumin filtration, thereby improving the safety profile of CRRT filters.


Assuntos
Terapia de Substituição Renal Contínua , Humanos , Habitação , Filtração , Ultrafiltração , Albuminas , Terapia de Substituição Renal/métodos
13.
BMC Plant Biol ; 23(1): 121, 2023 Mar 02.
Artigo em Inglês | MEDLINE | ID: mdl-36859186

RESUMO

It is crucial to understand how targeted traits in a hybrid breeding program are influenced by gene activity and combining ability. During the three growing seasons of 2015, 2016, and 2017, a field study was conducted with twelve cotton genotypes, comprised of four testers and eight lines. Thirty-two F1 crosses were produced in the 2015 breeding season using the line x tester mating design. The twelve genotypes and their thirty-two F1 crosses were then evaluated in 2016 and 2017. The results demonstrated highly significant differences among cotton genotypes for all the studied traits, showing a wide range of genetic diversity in the parent genotypes. Additionally, the line-x-tester interaction was highly significant for all traits, suggesting the impact of both additive and non-additive variations in gene expression. Furthermore, the thirty-two cotton crosses showed high seed cotton output, lint cotton yield, and fiber quality, such as fiber length values exceeding 31 mm and a fiber strength above 10 g/tex. Accordingly, selecting lines and testers with high GCA effects and crosses with high SCA effects would be an effective approach to improve the desired traits in cotton and develop new varieties with excellent yield and fiber quality.


Assuntos
Gossypium , Melhoramento Vegetal , Egito , Fenótipo , Hábitos
14.
Materials (Basel) ; 16(5)2023 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-36903064

RESUMO

Aeolian sand flow is a major cause of land desertification, and it is prone to developing into a dust storm coupled with strong wind and thermal instability. The microbially induced calcite precipitation (MICP) technique can significantly improve the strength and integrity of sandy soils, whereas it easily leads to brittle destruction. To effectively inhibit land desertification, a method coupled with MICP and basalt fiberreinforcement (BFR) was put forward to enhance the strength and toughness of aeolian sand. Based on a permeability test and an unconfined compressive strength (UCS) test, the effects of initial dry density (ρd), fiber length (FL), and fiber content (FC) on the characteristics of permeability, strength, and CaCO3 production were analyzed, and the consolidation mechanism of the MICP-BFR method was explored. The experiments indicated that the permeability coefficient of aeolian sand increased first, then decreased, and subsequently increased with the increase in FC, whereas it exhibited a tendency to decrease first and then increase with the increase in FL. The UCS increased with the increase in the initial dry density, while it increased first and then decreased with the increase in FL and FC. Furthermore, the UCS increased linearly with the increase in CaCO3 generation, and the maximum correlation coefficient reached 0.852. The CaCO3 crystals played the roles of providing bonding, filling, and anchoring effects, and the spatial mesh structure formed by the fibers acted as a bridge effect to enhance the strength and brittle damage of aeolian sand. The findings could supply a guideline for sand solidification in desert areas.

15.
Ophthalmol Sci ; 3(1): 100214, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36275201

RESUMO

Objective: Seventy percent of Fuchs' endothelial corneal dystrophy (FECD) cases are caused by an intronic trinucleotide repeat expansion in the transcription factor 4 gene (TCF4). The objective of this study was to characterize the corneal subbasal nerve plexus and corneal haze in patients with FECD with (RE+) and without the trinucleotide repeat expansion (RE-) and to assess the correlation of these parameters with disease severity. Design: Cross-sectional, single-center study. Participants: Fifty-two eyes of 29 subjects with a modified Krachmer grade of FECD severity from 1 to 6 were included in the study. Fifteen of the 29 subjects carried an expanded TCF4 allele length of ≥ 40 cytosine-thymine-guanine repeats (RE+). Main Outcomes Measures: In vivo confocal microscopy assessments of corneal nerve fiber length (CNFL), corneal nerve branch density, corneal nerve fiber density (CNFD), and anterior corneal stromal backscatter (haze); Scheimpflug tomography densitometry measurements of haze in anterior, central, and posterior corneal layers. Results: Using confocal microscopy, we detected a negative correlation between FECD severity and both CNFL and CNFD in the eyes of RE+ subjects (Spearman ρ = -0.45, P = 0.029 and ρ = -0.62, P = 0.0015, respectively) but not in the eyes of RE- subjects. Additionally, CNFD negatively correlated with the repeat length of the expanded allele in the RE+ subjects (Spearman ρ = -0.42, P = 0.038). We found a positive correlation between anterior stromal backscatter and severity in both the RE+ and RE- groups (ρ = 0.60, P = 0.0023 and ρ = 0.44, P = 0.024, respectively). The anterior, central, and posterior Scheimpflug densitometry measurements also positively correlated with severity in both the RE+ and RE- groups (P = 5.5 × 10-5, 2.5 × 10-4, and 2.9 × 10-4, respectively, after adjusting for the expansion status in a pooled analysis. However, for patients with severe FECD (Krachmer grades 5 and 6), the posterior densitometry measurements were higher in the RE+ group than in the RE- group (P < 0.05). Conclusions: Loss of corneal nerves in FECD supports the classification of the TCF4 trinucleotide repeat expansion disorder as a neurodegenerative disease. Haze in the anterior, central, and posterior cornea correlate with severity, irrespective of the genotype. Quantitative assessments of corneal nerves and corneal haze may be useful to gauge and monitor FECD disease severity in RE+ patients.

16.
Anat Rec (Hoboken) ; 306(2): 437-445, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36054569

RESUMO

Reversed sexual dimorphism (RSD) in size is a deep issue in evolutionary biology. RSD in body mass and linear measures is pronounced in diurnal predatory bird species, especially in those that feed on other birds. Size differences between males and females in internal organs or systems, such as the appendicular musculature, are less well known. In this study, 14 muscles related to toe closure in the Eurasian sparrowhawk (Accipiter nisus), a bird-eating species, were selected for dissection and architectural measurement. The muscle mass (MM), physiological cross-sectional area (PCSA), and fiber length (FL) were compared between sexes to detect the possible presence and/or degree of RSD. The results revealed significant RSD in MM and PCSA and suggested a higher force-generating capacity in females than in males. In females, greater strength in M. tibialis cranialis, M. iliofibularis, and six digital flexors enabled them to capture and carry larger prey, whereas more massive development in M. abductor digiti II and M. abductor digiti IV provided their feet with greater dexterity to improve the effectiveness of grasping larger mobile prey and preventing escape during capture. Fiber length did not show RSD. Generally, males had longer relative and absolute fiber length, indicative of enhanced working range and speed of contraction that was advantageous when hunting small prey. The differences between the sexes in architectural design and the high degree of RSD in MM and PCSA are correlated with the bird-eating diet and prey size difference of this species.


Assuntos
Falcões , Animais , Feminino , Masculino , Falcões/fisiologia , Caracteres Sexuais , Músculo Esquelético/fisiologia , Aves
17.
Plant Sci ; 328: 111576, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36565935

RESUMO

Fiber growing inside the cotton bolls is a highly demandable product and its quality is key to the success of the textile industry. Despite the various efforts to improve cotton fiber staple length Pakistan has to import millions of bales to sustain its industrial needs. To improve cotton fiber quality Bacterial cellulose synthase (Bcs) genes (acsA, acsB) were expressed in a local cotton variety CEMB-00. In silico studies revealed a number of conserved domains both in the cotton-derived and bacterial cellulose synthases which are essential for the cellulose synthesis. Transformation efficiency of 1.27% was achieved by using Agrobacterium shoot apex cut method of transformation. The quantitative mRNA expression analysis of the Bcs genes in transgenic cotton fiber was found to be many folds higher during secondary cell wall synthesis stage (35 DPA) than the expression during elongation phase (10 DPA). Average fiber length of the transgenic cotton plant lines S-00-07, S-00-11, S-00-16 and S-00-23 was calculated to be 13.02% higher than that of the non-transgenic control plants. Likewise, the average fiber strength was found to be 20.92% higher with an enhanced cellulose content of 22.45%. The mutated indigenous cellulose synthase genes of cotton generated through application of CRISPR/Cas9 resulted in 6.03% and 12.10% decrease in fiber length and strength respectively. Furthermore, mature cotton fibers of transgenic cotton plants were found to have increased number of twists with smooth surface as compared to non-transgenic control when analyzed under scanning electron microscope. XRD analysis of cotton fibers revealed less cellulose crystallinity index in transgenic cotton fibers as compared to control fibers due to deposition of more amorphous cellulose in transgenic fibers as a result of Bcs gene expression. This study paved the way towards unraveling the fact that Bcs genes influence cellulose synthase activity and this enzyme helps in determining the fate of cotton fiber length and strength.


Assuntos
Celulose , Fibra de Algodão , Glucosiltransferases/genética , Gossypium/genética , Regulação da Expressão Gênica de Plantas
18.
Data Brief ; 45: 108618, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36426085

RESUMO

Natural fibers used as reinforcements or fillers for materials development greatly affect properties and performance of end-use applications. As a consequence of conditioning processes such as grinding and sieving, average fiber length varies significantly. It is thus necessary to estimate the length as statistical data distribution rather than a single mean value. This approach implies length measurement of a significant number of fibers; however, a very high number of data points requires not only long-time frames but also significative amount of work. To address these issues, this article details a facile methodology to measure the length of a large number of natural fibers of oil palm empty fruit bunch (OPEFB) together with a statistical analysis to verify the correspondence between theoretical distributions and experimental data. Moreover, further information related to spectrophotometric, physico-chemical, mechanical, thermal, and morphological characteristics of OPEFB fibers coming from oil palm cultivation in Ecuador are presented. The data will contribute to comprehensively and rigorously describe the overall effects of natural fiber lengths on material properties.

19.
Polymers (Basel) ; 14(22)2022 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-36433004

RESUMO

The properties of short-fiber-reinforced composites depend on the fiber length of the reinforcing fibers. This fiber length is typically influenced by processing to different extents. In this work, we investigate the influence of processing, i.e., the influence of residence time achieved via different dosing points in compounding, and the fiber content on the fiber length and mechanical properties of short-carbon-fiber-reinforced polypropylene. We found that, with increasing fiber content, the fiber length decreases from 900 to 300 µm after compounding and from 500 to 250 µm after injection molding. Additionally, a decrease in residence time in the compounder leads to an increase in the fiber length of approx. 300 µm compared to the longer residence time. This is later reduced by the injection molding step, but the longer fibers are still longer in the final molded test specimen, thus resulting in a 5-10% increased tensile strength and elastic modulus as well as an some increase in impact strength. As the injection molding step showed considerable fiber length reduction (down to 250 µm), further investigations of injection molding should be undertaken to preserve fiber length better for the increased performance of these composites.

20.
Front Bioeng Biotechnol ; 10: 1002731, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36277379

RESUMO

Muscle-driven simulations have been widely adopted to study muscle-tendon behavior; several generic musculoskeletal models have been developed, and their biofidelity improved based on available experimental data and computational feasibility. It is, however, not clear which, if any, of these models accurately estimate muscle-tendon dynamics over a range of walking speeds. In addition, the interaction between model selection, performance criteria to solve muscle redundancy, and approaches for scaling muscle-tendon properties remain unclear. This study aims to compare estimated muscle excitations and muscle fiber lengths, qualitatively and quantitatively, from several model combinations to experimental observations. We tested three generic models proposed by Hamner et al., Rajagopal et al., and Lai-Arnold et al. in combination with performance criteria based on minimization of muscle effort to the power of 2, 3, 5, and 10, and four approaches to scale the muscle-tendon unit properties of maximum isometric force, optimal fiber length, and tendon slack length. We collected motion analysis and electromyography data in eight able-bodied subjects walking at seven speeds and compared agreement between estimated/modelled muscle excitations and observed muscle excitations from electromyography and computed normalized fiber lengths to values reported in the literature. We found that best agreement in on/off timing in vastus lateralis, vastus medialis, tibialis anterior, gastrocnemius lateralis, gastrocnemius medialis, and soleus was estimated with minimum squared muscle effort than to higher exponents, regardless of model and scaling approach. Also, minimum squared or cubed muscle effort with only a subset of muscle-tendon unit scaling approaches produced the best time-series agreement and best estimates of the increment of muscle excitation magnitude across walking speeds. There were discrepancies in estimated fiber lengths and muscle excitations among the models, with the largest discrepancy in the Hamner et al. model. The model proposed by Lai-Arnold et al. best estimated muscle excitation estimates overall, but failed to estimate realistic muscle fiber lengths, which were better estimated with the model proposed by Rajagopal et al. No single model combination estimated the most accurate muscle excitations for all muscles; commonly observed disagreements include onset delay, underestimated co-activation, and failure to estimate muscle excitation increments across walking speeds.

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